Transitional cell carcinoma of urinary bladder with metastasis in lumbar vertebrae and spinal cord compression in an ocelot(Leopardus pardalis) / Carcinoma de células de transição de bexiga urinária com metástase em vértebras lombares e compressão de medula espinhal em uma jaguatirica (Leopardus pardalis)

This paper reports a case of nonpapillary and infiltrative transitional cell carcinoma (TCC) of the urinary bladder with metastasis of lumbar vertebrae and spinal cord compression in an adult female ocelot (Leopardus pardalis), from the Mato Grosso state, Brazil. The ocelot had pelvic limb paralysis and skin ulcers in the posterior region of the body and was submitted to euthanasia procedure. At necropsy was observed a multilobulated and irregular shaped, yellowish to white nodule in the urinary bladder. The nodule had a soft consistency and arised from the mucosa of the urinary bladder extending throughout the muscular layers and the serosa. Nodules of similar appearance infiltrating the vertebral column the at L6 and L7 vertebrae with corresponding spinal canal invasion were also observed. The histological evaluation showed epithelial neoplastic proliferation in the urinary bladder with characteristics of nonpapillary and infiltrative TCC, with positive immunohistochemical staining for pancytokeratin, and strong immunostaining for cytokeratin of low molecular weight, and weak or absent labeling for high molecular weight cytokeratin. This is the first report of TCC of urinary bladder in ocelot in Brazil...

O presente trabalho relata um caso de carcinoma de células de transição (TCC) da bexiga urinária com metástase em vértebras lombares e compressão da medula espinhal em uma jaguatirica fêmea adulta (Leopardus pardalis), no estado do Mato Grosso. A jaguatirica manifestava paralisia de membros pélvicos e úlceras de decúbito na região posterior do corpo e foi submetida a eutanásia. Na necropsia observou-se um nódulo multilobulado e irregular, brancoamarelado na bexiga urinária, de consistência macia com origem na mucosa vesical e que se estendia às camadas musculares e à serosa. Nódulos de aspecto semelhante infiltrando as vértebras L6 e L7 e invasão do canal vertebral correspondente. No exame histológico foi observada proliferação neoplásica na bexiga urinária com com características de TCC não papilar e infiltrativo, com marcação imuno-histoquímica positiva para pancitoqueratina, forte marcação para citoqueratina de baixo peso molecular e fraca ou inexistente para citoqueratina de alto peso molecular. Este é o primeiro relato de TCC em bexiga urinária de jaguatirica no Brasil...

Antibody kinetics in goats and conceptuses naturally infected with Neospora caninum.

Neospora caninum is a protozoan which can cause abortions in caprines. However, information regarding the humoral immune response and the occurrence of reproductive disorders is scarce. This is the first study in which the kinetics of antibodies is studied in pregnant goats naturally infected by N. caninum, as well as their respective conceptuses. The subclasses of IgG (IgG1 and IgG2) were also evaluated in pregnant goats. Reproductive problems related to neosporosis (abortion and stillbirth) occurred in 15.38% of the goats. There was a statistically significant association between the increased titres of maternal IgG in the second half of the gestational period with the occurrence of endogenous transplacental transmission. The rate of congenital transmission was 77%. During the gestational period of the seropositive goats, there was mainly a predominance of the subclass IgG2, although mixed patterns of IgG2-IgG1 and the IgG1 pattern were also observed. These results indicate that N. caninum is responsible for the occurrence of important alterations in the humoral immune response of naturally infected goats, and is also a potential causative agent for reproductive disorders in goats. The high proportion of infected conceptuses reinforces the suggestion that congenital infection is one of the main routes of parasite transmission in goats.